|Yi Jing published an article on nucleoli protein NPM1 induction nucleoli stress on Nature Communications|
From Basic medical college of Shanghai jiaotong university department of cell biology, biochemistry and molecular science, Yi Jing and her research group published in the latest issue of the international academic journal Nature Communications (Nature Communications) (impact factor 11.3) online. This work is done with the team from the east China university of science and technology Yi Yang and a number of partners using REDOX probe and living cell imaging technology research nucleoli protein NPM1 induction nucleoli stress.
Nucleolus is synthesis and assembly ribosomes in the nuclei of place, in the classical concept of cell biology which is only responsible for the biosynthesis of ribosomes. Nearly a decade of subcellular proteomics found large amounts of protein in nucleoli and nuclear quality between shuttle shift, for unknown reasons. As were reported at the same time, many types of stress can cause the shift of nucleolus protein and activate the famous stress signaling pathways p53 pathway, causing the so-called “nucleoli stress (nucleolar stress)”. The nucleolus stress activated p53 has a variety of explanations. As for why all sorts of cell stress can show the nucleolus stress, there is no common sense.
Yi Jing and her team tended solve this problem, and at the same time in living cells expressed REDOX ro - GFP fluorescence probe and a fluorescent tag called nuclear phosphoprotein (NPM1) the nucleolus of protein, and real time observation and other methods.In the same cells, it was found that all kinds of different stimuli such as hunger, radiation, high temperature, low oxygen and chemotherapy drugs actinomycin D like hydrogen peroxide cause nucleoli zone of oxidation, triggering NPM1 from nucleolus shift to nuclear mass. Articles by looking in the same living cells wild type and mutant NPM1 shift model and observation of NPM1 alone or with oxidation catalytic enzyme protein displacement mode, which has been clear about the NPM1 one and a half cystine residues on C275 oxidation (type of GSH) modification leads to the protein and the nucleolus of rDNA and rRNA dissociation.It leaves the nucleolus into the nuclear mass. Finally it proved that although previous nucleoli, which put forward the activation of p53 stress related proteins involved in the incident indeed, but, if you don‘t shift NPM1 in nucleolus, as the article produced by the mutant NPM1, nucleolus stress is unable to cause p53 activation, so as to clarify the previous mentioned multiple molecules and NPM1 in mediating the role of p53 activation. Because the paper with real-time observed the nucleolus of oxidation and nucleoli protein oxidation and then shift to explain the nucleolus stress perception and response process, the topic of paper called “nuclear phosphoprotein induction nucleoli REDOX mechanism of stress (A REDOX mechanism physicist nucleolar stress sensing by nucleophosmin)”. Recently reported the role of chemotherapy drugs actinomycin D leukemia need combined with NPM1 C275. Therefore, nucleolus REDOX mechanism of stress induced not only provide clues for the understanding of the new function of nucleoli, which may also explained the action principle of chemotherapy drugs related to leukemia.
The first author isdoctor Yang Kai. Yang jie and Yi Jing are common corresponding authors, with the help of the east China university of science and technology, Chinese academy of sciences, and Zhou Aiwu from SJUSM etc. This research is sponsored by the national natural science fund and key project and the ministry of science and technology on the surface of 973 project funding.
The full text link: http://www.nature.com/articles/ncomms13599